The Microscopy Imaging & Cytometry Resources Core offers a variety of services.....

• Confocal Microscopy, including both point scanning (Zeiss LSM 510, Zeiss LSM 780, Leica TCS SP5 and spinning disk Zeiss Cell Observer Spinning Disk Microscope.
• Multiphoton Microscopy
• Fluorescent Microscopy
       Conventional, widefield epifluorescence,  (Zeiss Axiovert 200)
       Conventional, widefield epifluorescence with electronic stage (Leica DMi8)
• High through-put microplate imaging with the (Incucyte Live Cell Imaging System)
• Electron Microscopy with the Zeiss GeminiSEM 300 Block Face EM system, capable of SEM, TEM, Array Tomography, and Block Face imaging
• A host of EM support services, includiing sample embedding, trimming, sectioning for traditional EM and array tomography, block face sample mounting and loading, and EM image analysis
• In vivo small animal imaging
       In vivo small animal Positron Emission Tomography (PET) imaging
       In vivo small animal Single Photon Emission Computed Tomography (SPECT/CT) imaging
       In vivo small animal X-Ray imaging; In vivo small animal white light fluorescence and bioluminescence imaging (Carestream In Vivo Xtreme, Carestream MS FX PRO)
• Long-term, live cell imaging. All confocal microscopes are outfitted with live cell imaging chambers to control CO2 levels, temperature, and humidity
• Advanced Image Analysis, including 3D and 4D image reconstruction and quantitative measurements utilizing Huygens Scientific Volume Imaging and Volocity
• Cell sorting (operator driven), using the Sony SH800 or the SY3200. The SY3200 sorts up to 70,000 events/second and can sort up to 4 parameters simultaneously
• Cell analysis for data acquired using the benchtop cytometers and the Sony SY3200. These services range from simple one-color analysis of the cell cycle to multi-color, multi-laser analysis requiring expert knowledge of machine setup and color compensation
• Cytometry Data analysis, using any of three offline workstations in the Hudson Webber Cancer Research Center (HWCRC) or one in the Prentis Cancer Research Building that provides access to licensed Cellquest, Sony and FlowJo software for basic analysis and programs such as Modfit for cell cycle analysis. The researcher can undertake analysis after suitable training, or Core personnel will provide the service
• Expert consultation, including advide on the choice of application and experimental design -- contact us here
• Training is provided for every new user as well as refresher training for more experienced users, and workshops are provided, as announced
• On site support for use of equipment