The BD FACSCanto II is an 8-color, 3-laser analytical cytometer capable of handling most popular flow cytometry experiments, like the annexin V apoptosis assay.

The fixed optical configuration for the Canto II (with example fluorochromes):

• Violet (405nm): V450 (BV421), V525 (BV510)
• Blue (488nm): B530 (FITC), B585 (PE), B670 (PerCP-Cy5.5), B780 (PE-Cy7)
• Red (633nm): R660 (APC), R780 (APC-Cy7)