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Wayne State University

Aim Higher

School of Medicine

Services

The Microscopy Imaging & Cytometry Resources Core offers a variety of services.....

  • Confocal Microscopy, including both point scanning (Zeiss LSM 510, Zeiss LSM 410, Zeiss LSM 780, Leica TCS SP5 and spinning disk Zeiss Cell Observer Spinning Disk Microscope.
  • Multiphoton Microscopy, including Second Harmonic Generation imaging
  • Fluorescent Microscopy
         Conventional, widefield epifluorescence,  (Zeiss ApoTome)
         Optical sectioning using Structured Illumination, both upright and inverted stands (Zeiss ApoTome)
  • In vivo small animal imaging
    ​     In vivo small animal Positron Emission Tomography (PET) imaging
         In vivo small animal Single Photon Emission Computed Tomography (SPECT/CT) imaging
         In vivo small animal X-Ray imaging; In vivo small animal white light fluorescence and bioluminescence imaging (Carestream In Vivo Xtreme, Carestream MS FX PRO)
  • Long-term, live cell imaging. All confocal microscopes are outfitted with live cell imaging chambers to control CO2 levels, temperature, and humidity
  • Advanced Image Analysis, including 3D and 4D image reconstruction and quantitative measurements
  • Cell analysis for data acquired using the benchtop cytometers and the Sony SY3200. These services range from simple one-color analysis of the cell cycle to multi-color, multi-laser analysis requiring expert knowledge of machine setup and color compensation
  • Cell sorting (operator driven), using the Sony SH800 or the SY3200. The SY3200 sorts up to 70,000 events/second and can sort up to 4 parameters simultaneously.
  • Cytometry Data analysis, using any of three offline workstations in the Hudson Webber Cancer Research Center (HWCRC) or one in the Prentis Cancer Research Building that provides access to licensed Cellquest, Sony and FlowJo software for basic analysis and programs such as Modfit for cell cycle analysis. The researcher can undertake analysis after suitable training, or Core personnel will provide the service.
  • Expert consultation, including advide on the choice of application and experimental design -- contact us here
  • Training is provided for every new user as well as refresher training for more experienced users, and workshops are provided, as announced
  • On site support for use of equipment