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Wayne State University

Aim Higher

School of Medicine

Resources

General Microscopy Information:

Spectra Viewers:

Fluorochromes:

Flow Cytometry Resources:

Tools

  • FluoroFinder streamlines complex multicolor experiment design process down to three easy steps. Simply select your facility's cytometers, set your markers, and then choose the best available reagents for your criteria.
  • Chromocyte  a free web-based tool featuring a full contingent of variables for panel design, a reagent database, forum, and more
  • BD Biosciences Multicolor Flow Cytometry Tools  helpful resources for designing your flow experiments, fluorochrome specifications, FACSDiva primers, and more  
  • Life Technologies' Fluorescence Tutorials  informative tutorials covering the basics of fluorescence spectra, optics, flow cytometry and data analysis
  • Beckman Coulter Flow Cytometry Page  links to various educational resources and webinars, as well as product and instrument information
  • BioLegend Multicolor Panel Selector  an online tool for constructing an optimized flow panel

Protocols & References

  • Current Protocols in Cytometry  a "best practices" collection that distills and organizes the absolute latest techniques from the top cytometry labs and specialists worldwide. It is the most complete set of peer-reviewed protocols for flow and image cytometry available."
  • OMIPs  (Optimized Multicolor Immunofluorescence Panels)  a publication type in Cytometry Part A to communicate complex reagent panels for interrogating phenotypic and functional characteristics of cell populations
  • MIFlowCyt  (Minimum Information about a Flow Cytometry experiment)  a standard for outlining the minimum information required to report the experimental details of flow cytometry experiments
  • Practical Flow Cytometry  a reference book by Howard M. Shapiro
  • Compensation Basics  a primer on compensation by Mario Roederer: "... probably the least understood process accompanying flow cytometric anayses... compensation is absolutely crucial for some aspects of flow cytometry"
  • Flow Cytometry - A Basic Introduction  by Dr. Michael Ormerod is available as an online book from De Novo Software. It is designed for those new to flow cytometry; hard copy also available for purchase

Tutorials